New synthesis and biodistribution of the D-amino acid oxidase-magnetic nanoparticle system
نویسندگان
چکیده
BACKGROUND Application of nanoenzymes, based on D-amino acid oxidase (DAAO) conjugated to magnetic nanoparticles (NPs), as anticancer system requires improvement of the synthesis protocol and in vivo distribution evaluation. RESULTS A new and more efficient synthesis via EDC-NHS produced an Fe3O4NP-APTES-DAAO system with a specific activity of 7 U/mg NPs. IR spectroscopy showed that all Fe3O4 NP sites are saturated with APTES and all available NH2 sites with DAAO. The acute cytotoxicity of the new system does not differ from that of the previous one. In vivo experiments showed that the system did not cause adverse effects, cross the brain-blood barrier and accumulate in the heart. CONCLUSIONS Our results support the possibility to use enzymes conjugated to magnetic NPs for cancer treatment. Besides, we think that enzymes and other biological molecules efficiently conjugated to magnetic NPs might constitute a category of 'bionanoparticles' to be exploited, not only in medical, but also in industrial biotechnology.
منابع مشابه
Synthesis of novel amino-coated Fe3O4 magnetic nanoparticle functionalized with ciprofloxacin (AF-Fe3O4-NP@cpf) and study of its effect on Cu(II) and Ni(II) adsorption
Ciprofloxacin (1-cyclopropyl-6-fluoro-1, 4-dihydro-4-oxo-7-(1- piperazinyl) - 3-quinoline carboxylic acid, cpfH) is a member of synthetic antibacterial agent widely used in clinical practice for the treatment of various gram negative and positive microorganisms. In concurrent use of ciprofloxacin with cations, they may bind together and result to formation complex by chelating. Formation of che...
متن کاملCu-phthalocyanine Coated Hybrid Magnetic Nanoparticles: Preparation and Application in the Synthesis of Mono- and Bis pyrano[2,3-d]pyrimidinones and mono- and Bis-2-amino-4H-pyrans
In this research, Cu-phthalocyanine coated hybrid magnetic nanoparticles have been prepared in a simple method and evaluated as an efficient catalyst in the preparation of mono- and bis-pyrano[2,3-d]pyrimidinones and mono- and bis-2-amino-4H-pyrans from the condensation reaction of 1,3-dimethylbarbituric acid or 4-hydroxycumarin with malononitrile and mono- and bis-aldehydes under ultrasoni...
متن کاملFe3O4@SiO2@Am-PPC-SO3H] [HSO4]: A new magnetic solid acid nanocatalyst for the synthesis of benzoxazole derivatives
A novel magnetite nanoparticle-based heterogeneous acidic catalyst [Fe3O4@SiO2@Am-PPC-SO3H] [HSO4] was successfully prepared and characterized by SEM, EDX, TGA, VSM and FT-IR techniques. The magnetically retrievable and sustainable catalyst was investigated in the reaction of aldehyde and o-aminophenol for the synthesis of benzoxazoles. The...
متن کاملFe3O4@SiO2@Am-PPC-SO3H] [HSO4]: A new magnetic solid acid nanocatalyst for the synthesis of benzoxazole derivatives
A novel magnetite nanoparticle-based heterogeneous acidic catalyst [Fe3O4@SiO2@Am-PPC-SO3H] [HSO4] was successfully prepared and characterized by SEM, EDX, TGA, VSM and FT-IR techniques. The magnetically retrievable and sustainable catalyst was investigated in the reaction of aldehyde and o-aminophenol for the synthesis of benzoxazoles. The...
متن کاملMagnetic nanoparticle immobilized N-propylsulfamic acid: The efficient, green and reusable nanocatalyst for the synthesis of substituted coumarins
N-Propylsulfamic acid supported onto magnetic Fe3O4 nanoparticles (MNPs-PSA) was investigated as an efficient and magnetically recoverable catalyst for the one-pot synthesis of substituted coumarins from the reaction of coumarin with variety aromatic aldehydes in high to excellent yield at room temperature under solvent-free conditions. The magnetic nanocatalyst can be readily recovered easily ...
متن کامل